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A super repressor mutation (IS) results in:


A) no transcription.
B) inducible transcription.
C) transcription but no translation.
D) no translation.
E) constitutive transcription.

F) A) and D)
G) A) and E)

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A partial diploid of genotype I+ P+ O+ Z+ Y- / I+ P- O+ Z+ Y+ will show:


A) inducible production of β\beta -galactosidase.
B) inducible production of β\beta -galactosidase and permease.
C) constitutive production of β\beta -galactosidase.
D) constitutive production of β\beta -galactosidase and permease.
E) no β\beta -galactosidase or acetylase production at all.

F) A) and B)
G) A) and C)

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Which of the following is an example of a cis-acting element?


A) β\beta -galactosidase
B) operator site
C) lacI repressor protein
D) lactose
E) permease

F) All of the above
G) A) and E)

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Gene regulation in bacteria and bacteriophage utilize the following terminology.Twelve terms are given below (a - l) , followed by twelve statements.Write the letter of the correct term.A single letter is sufficient.Not all letters need be used.Some letters may be used more than once. Term List -phenotype of trp R+ O+ S+ in the presence of tryptophan


A) temporal control
B) positive control
C) negative control
D) inducible
E) noninducible
F) repressible
G) constitutive
H) operator
I) repressor
J) effector molecule
K) feedback inhibition
L) suppressor mutation

M) C) and K)
N) C) and E)

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In the presence of abundant tryptophan:


A) the attenuator stem-loop structure forms,allowing transcription to continue.
B) the attenuator stem-loop structure forms,terminating transcription.
C) the preemptor stem-loop structure forms,allowing transcription to continue.
D) the preemptor stem-loop structure forms,terminating transcription.
E) None of the answer options are correct.

F) B) and D)
G) D) and E)

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RNA polymerase binds to the promoter and transcribes the ara operon.In the presence of arabinose,both the CAP-cAMP complex and the AraC-arabinose complex must bind to:


A) araO.
B) araI.
C) araA.
D) araB.
E) araD.

F) C) and D)
G) All of the above

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B

Consider an E.coli cell with the following mutations.What effect would each mutation have on the function of the lac operon (assuming no glucose is present)? a)a mutant lac operator that cannot bind repressor b)a mutant lac repressor that cannot bind the lac operator c)a mutant lac repressor that cannot bind to allolactose d)a mutant lac promoter that cannot bind CAP plus cAMP

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a)The lac operon would always be turned ...

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In the presence of the repressor molecule and free tryptophan,the trp operon is:


A) constitutively transcribed.
B) derepressed.
C) induced.
D) repressed.
E) transcribed but not translated.

F) C) and D)
G) A) and D)

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D

The enzyme β\beta -galactosidase can convert the disaccharide lactose into:


A) allolactose.
B) glucose.
C) allolactose and glucose.
D) allolactose,glucose,and galactose.
E) sucrose and glucose.

F) D) and E)
G) A) and B)

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A prokaryotic operon is composed of a series of adjacent genes under the control of:


A) the same promoter.
B) the same operator.
C) an inducible promoter.
D) the same operator and promoter.
E) None of the answer options are correct.

F) A) and D)
G) A) and E)

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The repression of the transcription of lactose-metabolizing genes in the presence of glucose is an example of:


A) catabolite induction.
B) anabolite induction.
C) anabolite repression.
D) catabolite repression.
E) None of the answer options are correct.

F) C) and E)
G) B) and E)

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Which of the following is an example of a trans-acting factor?


A) β\beta -galactosidase
B) operator site
C) lacI repressor protein
D) lactose
E) permease

F) A) and D)
G) A) and E)

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Gene regulation in bacteria and bacteriophage utilize the following terminology.Twelve terms are given below (a - l) , followed by twelve statements.Write the letter of the correct term.A single letter is sufficient.Not all letters need be used.Some letters may be used more than once. Term List -type of operons responding to catabolite repression (glucose effect)


A) temporal control
B) positive control
C) negative control
D) inducible
E) noninducible
F) repressible
G) constitutive
H) operator
I) repressor
J) effector molecule
K) feedback inhibition
L) suppressor mutation

M) B) and G)
N) A) and I)

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Which of the following is/are NOT part of the trp operon?


A) trpR
B) trpE
C) trpC
D) trpA
E) All (trpR,trpE,trpC,and trpA) are part of the trp operon.

F) A) and E)
G) C) and E)

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Under conditions where tryptophan is scarce:


A) the attenuator stem-loop structure forms,allowing transcription to continue.
B) the attenuator stem-loop structure forms,terminating transcription.
C) the preemptor stem-loop structure forms,allowing transcription to continue.
D) the preemptor stem-loop structure forms,terminating transcription.
E) None of the answer options are correct.

F) A) and D)
G) D) and E)

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The order of the structural genes controlling the trp operon is:


A) trpE,trpD,trpC,trpB,trpA.
B) trpA,trpC,trpB,trpD,trpE.
C) trpE,trpD,trpC,trpA trpB.
D) trpA,trpB,trpC,trpD,trpE.
E) None of the answer options are correct.

F) C) and D)
G) A) and C)

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In Bacillus,the presence of alternate forms of which molecule is responsible for the control of large numbers of genes?


A) DNA dependent RNA polymerase
B) TFII
C) σ factor
D) Trp repressor
E) Operator protein

F) D) and E)
G) A) and B)

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Gene regulation in bacteria and bacteriophage utilize the following terminology.Twelve terms are given below (a - l) , followed by twelve statements.Write the letter of the correct term.A single letter is sufficient.Not all letters need be used.Some letters may be used more than once. Term List -small molecules that bind to regulatory molecule,such as repressor


A) temporal control
B) positive control
C) negative control
D) inducible
E) noninducible
F) repressible
G) constitutive
H) operator
I) repressor
J) effector molecule
K) feedback inhibition
L) suppressor mutation

M) I) and J)
N) A) and I)

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J

A constitutive operator mutation (Oc) results in:


A) no transcription.
B) inducible transcription.
C) transcription but no translation.
D) no translation.
E) constitutive transcription.

F) B) and D)
G) B) and E)

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Which of the following DNA-binding proteins contain a helix-turn-helix domain?


A) LacI repressor
B) TrpR repressor
C) AraC activator
D) λ\lambda and Cro repressor
E) All of the answer options are correct.

F) A) and B)
G) All of the above

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