Question 1

Which of the following statements is CORRECT about scanning electron microscopes?

Accepted Answer

A)   Thin segments must be used.
B)   The sample is placed in a vacuum.
C)   Stains can be applied to improve contrast.
D)   Images of surface structures are produced.
E)   Glass lenses are used.F) C) and D)
G) C) and E)

Question 2

An integral part of serological testing is the use of a solution called (blood/plasma/antiserum) that contains antibodies.

Accepted Answer

The answer of An integral part of serological testing is...

Question 3

A measurement of a microbe is reported as 1 × 10-6 m, also known as

Accepted Answer

A)   micrometers (μm) .
B)   yards.
C)   nanometers (nm) .
D)   millimeters (mm) .
E)   centimeters (cm) .F) C) and E)
G) A) and B)

Question 4

List and explain five types of techniques that can be used to identify unknown microorganisms.

Accepted Answer

There are five major types of identifica...

Question 5

A(n) (acidic/metallic/fluorescent) molecule is one that absorbs invisible radiation and emits visible light.

Accepted Answer

The answer of A(n) (acidic/metallic/fluorescent) molecule is one that absorbs...

Question 6

A virologist wants to observe the surface features of virus particles she is studying. Which of the following microscopes would NOT be useful for her observations?

Accepted Answer

A)   transmission electron
B)   scanning electron
C)   atomic force
D)   scanning tunneling
E)   differential interference contrastF) C) and E)
G) A) and C)

Question 7

Why are modern light microscopes better than the ones Leeuwenhoek used?

Accepted Answer

A)   Modern microscopes have a fivefold better resolution.
B)   Modern microscopes have lenses with smaller numerical apertures.
C)   Modern lenses are made of prisms.
D)   Modern microscopes are compound and have fivefold better resolution.
E)   Modern microscopes are compound instead of simple.F) C) and D)
G) B) and E)

Question 8

The process of immobilizing organisms on a glass slide through the application of either heat or chemicals is (staining/mordant/fixation).

Accepted Answer

The answer of The process of immobilizing organisms on a...

Question 9

Light rays that pass through the edge of a curved lens will be refracted more than those that pass through the center.

Accepted Answer

A) True 
 B)False

Question 10

Stains for light microscopy contain chromophores whereas stains for electron microscopy contain heavy metals.

Accepted Answer

A) True 
 B)False

Question 11

The most appropriate unit of measurement for intact archaea is the

Accepted Answer

A)   nanometer (nm) .
B)   micrometer (μm) .
C)   millimeter (mm) .
D)   meter (m) .
E)   centimeter (cm) .F) B) and D)
G) B) and C)

Question 12

Which of the following statements is CORRECT regarding modern taxonomy?

Accepted Answer

A)   There is consensus about the definition of species.
B)   Modern classifications reflect evolutionary relationships of organisms.
C)   Modern taxonomy does not provide a basis for predicting structural relationships.
D)   The modern taxonomic organization is a finished product.
E)   It is a complete inventory of all living organisms.F) B) and D)
G) C) and D)

Question 13

Lasers are used to generate the images produced by microscopes.

Accepted Answer

A)   fluorescent
B)   atomic force
C)   confocal
D)   phase-contrast
E)   both confocal and atomic forceF) None of the above
G) A) and C)

Question 14

If a microbiology lab student left the safranin out of the Gram stain procedure, what would be the result?

Accepted Answer

A)   All cells would be pink.
B)   Gram-positive cells would be purple and Gram-negative cells would be colorless.
C)   Gram-positive cells would be colorless and Gram-negative cells would be pink.
D)   All cells would be purple.
E)   Gram-positive cells would be pink and Gram-negative cells would be purple.F) A) and D)
G) A) and C)

Question 15

All of the following are common to both the Gram stain and the acid -fast stain EXCEPT

Accepted Answer

A)   primary stain.
B)   a chemical mordant.
C)   counterstain.
D)   a decolorizing agent.
E)   a decolorizing agent and a counterstain.F) C) and E)
G) B) and E)

Question 16

Gram staining of bacteria provides all the physical characterization necessary to identify bacterial species.

Accepted Answer

A) True 
 B)False

Question 17

Bacteria and many other microbes do NOT and therefore do NOT fit Linnaeusʹ definition of species.

Accepted Answer

A)   exchange genetic material
B)   reproduce sexually
C)   reproduce asexually
D)   have nuclei
E)   have cytoplasmic membranesF) B) and E)
G) C) and D)

Question 18

In a compound microscope, the lens that directs light through the specimen is the (ocular/condenser/objective) lens.

Accepted Answer

The answer of In a compound microscope, the lens that...

Question 19

Coating a specimen with a heavy metal is a step in preparing it for (phase/fluorescent/electron) microscopy.

Accepted Answer

The answer of Coating a specimen with a heavy metal...

Question 20

A useful unit for measuring the size of most cells is

Accepted Answer

A)   nanometers.
B)   micrometers.
C)   decimeters.
D)   millimeters.
E)   centimeters.F) A) and C)
G) A) and D)

Exam 4: Microscopy, Staining, and Classification

An integral part of serological testing is the use of a solution called (blood/plasma/antiserum) that contains antibodies.

Correct AnswerverifiedShow Answer

Why are modern light microscopes better than the ones Leeuwenhoek used?

Correct AnswerverifiedShow Answer

Which of the following statements is CORRECT regarding modern taxonomy?

Correct AnswerverifiedShow Answer

If a microbiology lab student left the safranin out of the Gram stain procedure, what would be the result?

Correct AnswerverifiedShow Answer

Which of the following statements is CORRECT about scanning electron microscopes?

Correct AnswerverifiedShow Answer

The process of immobilizing organisms on a glass slide through the application of either heat or chemicals is (staining/mordant/fixation).

Correct AnswerverifiedShow Answer

List and explain five types of techniques that can be used to identify unknown microorganisms.

Correct AnswerverifiedThere are five major types of identifica...View AnswerShow Answer

Light rays that pass through the edge of a curved lens will be refracted more than those that pass through the center.

Correct AnswerverifiedShow Answer

A(n) (acidic/metallic/fluorescent) molecule is one that absorbs invisible radiation and emits visible light.

Correct AnswerverifiedShow Answer

A useful unit for measuring the size of most cells is

Correct AnswerverifiedShow Answer

In a compound microscope, the lens that directs light through the specimen is the (ocular/condenser/objective) lens.

Correct AnswerverifiedShow Answer

A virologist wants to observe the surface features of virus particles she is studying. Which of the following microscopes would NOT be useful for her observations?

Correct AnswerverifiedShow Answer

Gram staining of bacteria provides all the physical characterization necessary to identify bacterial species.

Correct AnswerverifiedShow Answer

All of the following are common to both the Gram stain and the acid -fast stain EXCEPT

Correct AnswerverifiedShow Answer

Coating a specimen with a heavy metal is a step in preparing it for (phase/fluorescent/electron) microscopy.

Correct AnswerverifiedShow Answer

Bacteria and many other microbes do NOT and therefore do NOT fit Linnaeusʹ definition of species.

Correct AnswerverifiedShow Answer

A measurement of a microbe is reported as 1 × 10-6 m, also known as

Correct AnswerverifiedShow Answer

The most appropriate unit of measurement for intact archaea is the

Correct AnswerverifiedShow Answer

Lasers are used to generate the images produced by microscopes.

Correct AnswerverifiedShow Answer

Stains for light microscopy contain chromophores whereas stains for electron microscopy contain heavy metals.

Correct AnswerverifiedShow Answer

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There are five major types of identifica...
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